Microinjection pp 113-122 | Cite as

Bacteriophage φC31 Integrase Mediated Transgenesis in Xenopus laevis for Protein Expression at Endogenous Levels

  • Bryan G. Allen
  • Daniel L. Weeks
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 518)

Abstract

Bacteriophage φC31 inserts its genome into that of its host bacterium via the integrase enzyme which catalyzes recombination between a phage attachment site (attP) and a bacterial attachment site (attB). Integrase requires no accessory factors, has a high efficiency of recombination, and does not need perfect sequence fidelity for recognition and recombination between these attachment sites. These imperfect attachment sites, or pseudo-attachment sites, are present in many organisms and have been used to insert transgenes in a variety of species. Here we describe the φC31 integrase approach to make transgenic Xenopuslaevis embryos.

Key words

Xenopus φC31 integrase transgenesis fluorescence 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Bryan G. Allen
    • 1
  • Daniel L. Weeks
    • 1
  1. 1.Department of BiochemistryUniversity of IowaIowa CityUSA

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