Electroporation Protocols pp 351-359 | Cite as
Electroporation for Drug and Gene Delivery in the Clinic: Doctors Go Electric
Protocol
Abstract
Electroporation is a unique system for drug and gene delivery, as it is possible to very specifically target certain tissues within the body with whatever drug, gene, isotope, or other product is desired in a specific situation. An increasing number of clinical trials are being launched, and sophistication of equipment and protocols continues. This chapter reviews present knowledge from clinical trials, describes important issues in the patient management when using electroporation, and outlines future perspectives of the technology.
Keywords
electroporation clinical protocol electrode electrochemotherapy gene electrotransferNotes
Acknowledgements
The ESOPE study was supported by the European Union's 5th FP, under the leadership of Lluis M. Mir. Dr. Poul F. Geertsen photographed the treatment session shown in Fig. 27.1.
References
- 1.1. Jaroszeski, M., Heller, R., and Gilbert, R. (2000) Electrochemotherapy, electrogenetherapy, and transdermal drug delivery. Humana, Totowa, NJ.CrossRefGoogle Scholar
- 2.2. Belehradek, M., Domenge, C., Luboinski, B., et al. (1993) Electrochemotherapy, a new antitumor treatment. First clinical phase I-II trial. Cancer. 72, 3694–3700.CrossRefPubMedGoogle Scholar
- 3.3. Gilbert, R.A., Jaroszeski, M.J., and Heller, R. (1997) Novel electrode designs for electrochemotherapy. Biochim. Biophys. Acta. 1334, 9–14.PubMedGoogle Scholar
- 4.4. Gehl, J., Sørensen, T.H., Nielsen, K., et al. (1999) In vivo electroporation of skeletal muscle: Threshold, efficacy and relation to electric field distribution. Biochim. Biophys. Acta. 1428, 233–240.PubMedGoogle Scholar
- 5.5. Heller, R., Jaroszeski, M., Atkin, A., et al. (1996) In vivo gene electroinjection and expression in rat liver. FEBS Lett. 389, 225–228.CrossRefPubMedGoogle Scholar
- 6.6. Bureau, M.F., Gehl, J., Deleuze, V., Mir, L.M., and Scherman, D. (2000) Importance of association between permeabilization and electrophoretic forces for intramuscular DNA electrotransfer. Biochim. Biophys. Acta. 1474, 353–359.PubMedGoogle Scholar
- 7.7. Mir, L.M., Bureau, M.F., Gehl, J. et al. (1999) High efficiency gene transfer into skeletal muscle mediated by electric pulses. Proc. Natl. Acad. Sci. U.S.A. 96, 4262–4267.CrossRefPubMedGoogle Scholar
- 8.8. Heller, R., Jaroszeski, M.J., Glass, L.F. et al. (1996) Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer. 77, 964–971.CrossRefPubMedGoogle Scholar
- 9.9. Gehl, J. and Geertsen, P. (2000) Efficient palliation of hemorrhaging malignant melanoma skin metastases by electrochemotherapy. Melanoma Res. 10, 585–589.CrossRefPubMedGoogle Scholar
- 10.10. Marty, M., Sersa, G., Garbay, J.R., Gehl, J., Collins, C.G., Snoj, M., et al. (2006) Electrochemotherapy – An easy, highly effective and safe treatment of cutaneous and subcutaneous metastases: Results of ESOPE (European Standard Operating Procedures of Electrochemotherapy) study. Ejc Supplements. 4, 3–13.Google Scholar
- 11.11. Aihara, H. and Miyazaki, J.I. (1998) Gene transfer into muscle by electroporation in vivo. Nat. Biotechnol. 16, 867–870.CrossRefPubMedGoogle Scholar
- 12.12. Mir, L.M., Gehl, J., Sersa, G., Collins, C.G., Garbay, J.R., Billard, V., et al. (2006) Standard operating procedures of the electrochemotherapy: Instructions for the use of bleomycin or cisplantin administered either systemically or locally and electric pulses delivered by the Cliniporator (TM) by means of invasive or non-invasive electrodes. Ejc Supplements. 4, 14–25.Google Scholar
- 13.13. Gehl, J., Skovsgaard, T., and Mir, L.M. (2002) Vascular reactions to in vivo electroporation: characterization and consequences for drug and gene delivery. Biochim. Biophys. Acta. 1569, 51–58.PubMedGoogle Scholar
- 14.14. Sersa, G., Cemazar, M., Parkins, C.S., and Chaplin, D.J. (1999) Tumour blood flow changes induced by application of electric pulses. Eur. J. Cancer. 35, 672–677.CrossRefPubMedGoogle Scholar
- 15.15. Tsurumi, Y., Takeshita, S., Chen, D., et al. (1996) Direct intramuscular gene transfer of naked DNA encoding vascular endothelial growth factor augments collateral development and tissue perfusion. Circulation. 94, 3281–3290.PubMedGoogle Scholar
- 16.16. Takeshita, S., Isshiki, T., and Sato, T. (1996) Increased expression of direct gene transfer into skeletal muscles observed after acute ischemic injury in rats. Lab. Invest. 74, 1061–1065.PubMedGoogle Scholar
- 17.17. Orlowski, S., Belehradek, J., Jr., Paoletti, C., and Mir, L.M. (1988) Transient electropermeabilization of cells in culture. Increase of the cytotoxicity of anticancer drugs. Biochem. Pharmacol. 37, 4727–4733.CrossRefPubMedGoogle Scholar
- 18.18. Jaroszeski, M.J., Dang, V., Pottinger, C., Hickey, J., Gilbert, R., and Heller, R. (2000) Toxicity of anticancer agents mediated by electroporation in vitro. Anticancer Drugs. 11, 201–208.CrossRefPubMedGoogle Scholar
- 19.19. Gehl, J., Skovsgaard, T., and Mir, L.M. (1998) Enhancement of cytotoxicity by electropermeabilization: an improved method for screening drugs. Anticancer Drugs. 9, 319–325.CrossRefPubMedGoogle Scholar
- 20.20. Gothelf, A., Mir, L.M., and Gehl, J. (2003) Electrochemotherapy: results of cancer treatment using enhanced delivery of bleomycin by electroporation. Cancer Treat. Rev. 29, 371–387.CrossRefPubMedGoogle Scholar
- 21.21. Sersa, G., Cemazar, M., and Miklavcic, D. (1995) Antitumor effectiveness of electrochemotherapy with cis-diamminedichloroplatinum(II) in mice. Cancer Res. 55, 3450–3455.PubMedGoogle Scholar
- 22.22. Sersa, G., Cemazar, M., and Rudolf, Z. (2003) Electrochemotherapy: advantages and drawbacks in treatment of cancer patients. Cancer Ther. 1, 133–142.Google Scholar
- 23.23. Mir, L.M., Moller, P.H., Andre, F., and Gehl, J. (2005) Electric pulse-mediated gene delivery to various animal tissues. Adv. Genet. 54, 83–114.CrossRefPubMedGoogle Scholar
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