Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation
Histone chaperones are a group of histone-interacting proteins, involved in several important cellular functions. These chaperones are essential to facilitate ordered assembly of nucleosomes, both in replication dependent and independent manner. Replication independent function of histone chaperone is necessary for histone eviction during transcriptional initiation and elongation. In this chapter we have discussed a method to evaluate the role of histone chaperone NPM1 (the only known chaperone to get acetylated with functional consequence) in the transcriptional activation which is acetylation dependent.
Key wordsNPM1 acetylation nucleosomes transcriptional co-activators histone transfer assay
We thank R. G. Roeder and J. Kadonaga for some of the valuable reagents used in this work.
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