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Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation

  • Shrikanth S. Gadad
  • Jayasha Shandilya
  • Venkatesh Swaminathan
  • Tapas K. Kundu
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 523)

Abstract

Histone chaperones are a group of histone-interacting proteins, involved in several important cellular functions. These chaperones are essential to facilitate ordered assembly of nucleosomes, both in replication dependent and independent manner. Replication independent function of histone chaperone is necessary for histone eviction during transcriptional initiation and elongation. In this chapter we have discussed a method to evaluate the role of histone chaperone NPM1 (the only known chaperone to get acetylated with functional consequence) in the transcriptional activation which is acetylation dependent.

Key words

NPM1 acetylation nucleosomes transcriptional co-activators histone transfer assay 

Notes

Acknowledgments

We thank R. G. Roeder and J. Kadonaga for some of the valuable reagents used in this work.

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Shrikanth S. Gadad
    • 1
  • Jayasha Shandilya
    • 1
  • Venkatesh Swaminathan
    • 2
  • Tapas K. Kundu
    • 1
  1. 1.Transcription and Disease Laboratory, Molecular Biology and Genetics UnitJawaharlal Nehru Centre for Advanced Scientific ResearchJakkurIndia
  2. 2.Workman LabStowers Institute for Medical ResearchKansas CityUSA

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