SARS- and Other Coronaviruses pp 319-329

Part of the Methods in Molecular Biology book series (MIMB, volume 454)

Identification of Sugar Residues Involved in the Binding of TGEV to Porcine Brush Border Membranes

  • Christel Schwegmann-Wessels
  • Georg Herrler

Abstract

Coronaviruses most often infect the respiratory or intestinal tract. Transmissible gastroenteritis virus (TGEV), a group 1 coronavirus, infects the porcine small intestine. Piglets up to the age of 3 weeks die from diarrhea caused by the viral gastroenteritis unless they are protected by antibodies. In addition to the cellular receptor, porcine aminopeptidase N, the TGEV spike protein binds to sialic acid residues. We have shown that the sialic acid binding activity mediates the binding of TGEV to a mucin-like glycoprotein present in porcine brush border membranes. This was shown by performing a virus overlay binding assay with proteins obtained from brush border membranes by lectin precipitation. Because of the reactivity with specific lectins we assume that the recognized glycoprotein has the characteristics of a mucin.

Key Words

transmissible gastroenteritis virus sialic acid binding activity brush border membranes piglets lectin precipitation virus overlay binding assay 

Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Christel Schwegmann-Wessels
    • 1
  • Georg Herrler
    • 1
  1. 1.Institute for Virology, University of Veterinary Medicine HannoverHannoverGermany

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