Exocytosis and Endocytosis pp 15-33

Part of the Methods in Molecular Biology book series (MIMB, volume 440)

Pharmacological Inhibition of Endocytic Pathways: Is It Specific Enough to Be Useful?

  • Andrei I. Ivanov

Summary

Eukaryotic cells constantly form and internalize plasma membrane vesicles in a process known as endocytosis. Endocytosis serves a variety of housekeeping and specialized cellular functions, and it can be mediated by distinct molecular pathways. Among them, internalization via clathrin-coated pits, lipid raft/caveolae-mediated endocytosis and macropinocytosis/phagocytosis are the most extensively characterized. The major endocytic pathways are usually distinguished on the basis of their differential sensitivity to pharmacological/chemical inhibitors, although the possibility of nonspecific effects of such inhibitors is frequently overlooked. This review provides a critical evaluation of the selectivity of the most widely used pharmacological inhibitors of clathrin-mediated, lipid raft/caveolae-mediated endocytosis and macropinocytosis/phagocytosis. The mechanisms of actions of these agents are described with special emphasis on their reported side effects on the alternative internalization modes and the actin cytoskeleton. The most and the least-selective inhibitors of each major endocytic pathway are highlighted.

Keywords

Caveolae clathrin lipid rafts macropinocytosis phagocytosis selectivity 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Andrei I. Ivanov
    • 1
  1. 1.Department of Medicine, Gastroenterology and Hepatology DivisionUniversity of Rochester School of Medicine and DentistryRochesterUSA

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