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Nuclear Magnetic Resonance-Based Modeling and Refinement of Protein Three-Dimensional Structures and Their Complexes

  • Gloria Fuentes
  • Aalt D. J. van Dijk
  • Alexandre M. J. J. Bonvin
Protocol
Part of the Methods Molecular Biology™ book series (MIMB, volume 443)

Summary

Nuclear magnetic resonance (NMR) has become a well-established method to characterize the structures of biomolecules in solution. High-quality structures are now produced, thanks to both experimental and computational developments, allowing the use of new NMR parameters and improved protocols and force fields in structure calculation and refinement. In this chapter, we give a short overview of the various types of NMR data that can provide structural information, and then focus on the structure calculation methodology itself. We discuss and illustrate with tutorial examples both “classical” structure calculation and refinement approaches as well as more recently developed protocols for modeling biomolecular complexes.

Keywords

Docking NMR Refinement Structure calculation Validation of structures 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Gloria Fuentes
    • 1
  • Aalt D. J. van Dijk
    • 1
  • Alexandre M. J. J. Bonvin
    • 1
  1. 1.Bijvoet Center for Biomolecular ResearchUtrecht UniversityUtrechtThe Netherlands

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