The Mycobacterium tuberculosis Phagosome
Tuberculosis is currently the most devastating human bacterial disease, causing millions of deaths annually and infecting an overwhelming percentage of the global population. Its success as a scourge lies in the ability of Mycobacterium tuberculosis to prevent normal phagolysosome biogenesis, essential to the destruction of invading microorganisms, inside macrophages. Recent work has identified host GTPases involved in the block of normal phagolysosome biogenesis during mycobacterial infection and has provided a set of methods, in particular efficient macrophage transfection, which will prove essential in examining the role of host effectors in this process.
Key WordsM. tuberculosis phagosome Rabs maturation Rab conversion
- 3.Sturgill-Koszycki, S., Schaible, U. E., and Russell, D. G. (1996) Mycobacterium-containing phagosomes are accessible to early endosomes and reflect a transitional state in normal phagosome biogenesis. EMBO J. 15, 6960.Google Scholar
- 8.Kelley, V. A. and Schorey, J. S. (2003) Mycobacterium’s arrest of phagosome maturation in macrophages requires Rab5 activity and accessibility to iron. Mol. Biol. Cell 14, 3366.Google Scholar
- 9.Clemens, D. L., Lee, B. Y., and Horwitz, M. A. (2000) Mycobacterium tuberculosis and Legionella pneumophila phagosomes exhibit arrested maturation despite acquisition of Rab7. Infect. Immun. 68, 5154.Google Scholar
- 10.Clemens, D. L., Lee, B. Y., and Horwitz, M. A.. (2000) Deviant expression of Rab5 on phagosomes containing the intracellular pathogens Mycobacterium tuberculosis and Legionella pneumophila is associated with altered phagosomal fate. Infect. Immun. 68, 2671.Google Scholar
- 14.Kyei, G. B., Vergne, I., Chua, J., et al. (2006) Rab14 is critical for maintenance of Mycobacterium tuberculosis phagosome maturation arrest. EMBO J. 25, 5250.Google Scholar