The Mycobacterium tuberculosis Phagosome

  • Esteban A. Roberts
  • Vojo Deretic
Part of the Methods in Molecular Biology™ book series (MIMB, volume 445)


Tuberculosis is currently the most devastating human bacterial disease, causing millions of deaths annually and infecting an overwhelming percentage of the global population. Its success as a scourge lies in the ability of Mycobacterium tuberculosis to prevent normal phagolysosome biogenesis, essential to the destruction of invading microorganisms, inside macrophages. Recent work has identified host GTPases involved in the block of normal phagolysosome biogenesis during mycobacterial infection and has provided a set of methods, in particular efficient macrophage transfection, which will prove essential in examining the role of host effectors in this process.

Key Words

M. tuberculosis phagosome Rabs maturation Rab conversion 


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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Esteban A. Roberts
    • 1
  • Vojo Deretic
    • 2
  1. 1.Department of Molecular Genetics and MicrobiologyUniversity of New Mexico School of MedicineAlbuquerque
  2. 2.Health Sciences Center, Department Molecular Genetics and MicrobiologyUniversity of New Mexico Health Sciences CenterAlbuquerqueUSA

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