Analysis of Redox Relationships in the Plant Cell Cycle: Determinations of Ascorbate, Glutathione and Poly (ADPribose) Polymerase (PARP) in Plant Cell Cultures

  • Christine H. Foyer
  • Till K. Pellny
  • Vittoria Locato
  • Laura De Gara
Protocol
First Online:
Part of the Methods in Molecular Biology™ book series (MIMB, volume 476)

Abstract

Reactive oxygen species (ROS) and low molecular weight antioxidants, such as glutathione and ascorbate, are powerful signaling molecules that participate in the control of plant growth and development, and modulate progression through the mitotic cell cycle. Enhanced reactive oxygen species accumulation or low levels of ascorbate or glutathione cause the cell cycle to arrest and halt progression especially through the G1 checkpoint. Plant cell suspension cultures have proved to be particularly useful tools for the study of cell cycle regulation. Here we provide effective and accurate methods for the measurement of changes in the cellular ascorbate and glutathione pools and the activities of related enzymes such poly (ADP-ribose) polymerase during mitosis and cell expansion, particularly in cell suspension cultures. These methods can be used in studies seeking to improve current understanding of the roles of redox controls on cell division and cell expansion.

Keywords

Antioxidants plant cell suspension cultures cell cycle cellular redox state oxidative stress mitochondria cell death poly (ADPribose) polymerase 
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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Christine H. Foyer
    • 1
  • Till K. Pellny
    • 2
  • Vittoria Locato
    • 3
  • Laura De Gara
    • 3
  1. 1.School of Agriculture, Food and Rural DevelopmentNewcastle UniversityNewcastle upon TyneUK
  2. 2.Crop Performance and Improvement DivisionRothamsted ResearchHarpenden, HertsUK
  3. 3.Dipartimento di Biologia e Patologia VegetaleBariItaly

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