Protocol

Plant Pathology

Volume 508 of the series Methods in Molecular Biology pp 249-258

Date:

Direct Detection of Plant Viruses in Potato Tubers using Real-time PCR

  • Neil BoonhamAffiliated withCentral Science Laboratory, Sand Hutton Email author 
  • , Lynn LaurensonAffiliated withCentral Science Laboratory, Sand Hutton
  • , Rebecca WeekesAffiliated withCentral Science Laboratory, Sand Hutton
  • , Rick MumfordAffiliated withCentral Science Laboratory, Sand Hutton

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Summary

Virus indexing of seed potatoes can be carried out by growing eye plugs to produce small plants and then testing them by ELISA, but this method is time consuming. Direct testing of the eye plugs by ELISA is not reliable, and so a method has been developed for the routine testing of seed potatoes for virus by PCR.

Keywords:

Virus ELISA RT PCR Potatoes