Stem Cells in Regenerative Medicine pp 407-418

Part of the Methods in Molecular Biology book series (MIMB, volume 482)

Reprogramming of Liver to Pancreas

  • Shifaan Thowfeequ
  • Wan-Chun Li
  • Jonathan M.W. Slack
  • David Tosh

Abstract

Islet grafts have demonstrated that patients with diabetes would benefit greatly by β-cell therapy. However, the paucity of available islets for transplantation as well as the immunological barriers faced in allogeneic transplantation represent a tremendous barrier to regenerative approaches to the treatment of diabetes. Here, we present a strategy and protocols to transdifferentiate developmentally related hepatocytes into β-cells by the ectopic expression of critical β-cell transcription factors.

Key words

Two-step collagenase liver perfusion hepatocyte culture pancreatic transcription factors Pdx1 adenovirus transdifferentiation immunofluorescence 

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Copyright information

© Humana Press, a part of Springer Science+Business Media, LLC 2009

Authors and Affiliations

  • Shifaan Thowfeequ
    • 1
  • Wan-Chun Li
    • 1
  • Jonathan M.W. Slack
    • 1
  • David Tosh
    • 1
  1. 1.Centre for Regenerative Medicine, Department of Biology and BiochemistryUniversity of BathUK

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