Reprogramming of Liver to Pancreas
Islet grafts have demonstrated that patients with diabetes would benefit greatly by β-cell therapy. However, the paucity of available islets for transplantation as well as the immunological barriers faced in allogeneic transplantation represent a tremendous barrier to regenerative approaches to the treatment of diabetes. Here, we present a strategy and protocols to transdifferentiate developmentally related hepatocytes into β-cells by the ectopic expression of critical β-cell transcription factors.
Key wordsTwo-step collagenase liver perfusion hepatocyte culture pancreatic transcription factors Pdx1 adenovirus transdifferentiation immunofluorescence
- 3.Michalopoulos, G. K. (1990) Liver regeneration: molecular mechanisms of growth control. FASEB J. 14, 176–187.Google Scholar
- 7.Tollefson, A. E., Hermiston, T. W., and Wold, W. S. M. (1998) Preparation and titration of CsCl-banded adenovirus stock, in Adenovirus Methods and Protocols, Humana, Totowa, NJ.Google Scholar