Immobilization of Enzymes on Electrodes

  • Gilvanda Silva Nunes
  • Jean-Louis Marty
Part of the Methods in Biotechnology™ book series (MIBT, volume 22)


This chapter focuses on the four main immobilization techniques used in the development of enzyme electrodes: adsorption, entrapment, covalent coupling, and cross-linking. The performance of the immobilization method depends on the enzyme and each method has both advantages and drawbacks. Loss of the activity results from the change in enzyme conformation depending on the method of immobilization as well as modification of the microenvironment (i.e., local pH of the enzyme). The activity of the immobilized enzyme decreases as a result of barrier of diffusion, which slows down or prevents the substrate from reaching the active site of the enzyme. The development and the characteristics of an acetylcholinesterase biosensor are described

Key Words

Enzyme immobilization conformation microenvironment acetylcholinesterase biosensor 


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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Gilvanda Silva Nunes
    • 1
  • Jean-Louis Marty
    • 2
  1. 1.Technological Chemistry DepartmentCCET, Federal University of MaranhãoSão Lués, MaranhãoBrazil
  2. 2.Centre de PhytopharmacieUniversité de PerpignanPerpignan CedexFrance

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