Immobilization of Enzymes as the 21st Century Begins

An Already Solved Problem or Still an Exciting Challenge?
  • Jose M. Guisan
Protocol
Part of the Methods in Biotechnology™ book series (MIBT, volume 22)

Abstract

The main goal of enzyme immobilization is the industrial re-use of enzymes for many reaction cycles. In this way, simplicity and improvement of enzyme properties have to be strongly associated with the design of protocols for enzyme immobilization. In spite of their excellent catalytic properties, enzymes have many other characteristics that are not very suitable for their use in chemical industries: low stability, inhibition by high concentrations of substrates and products, low activity and selectivity toward nonnatural substrates under nonconventional conditions, and so on. The possibility of improving these unsuitable characteristics via the design of simple immobilization protocols is a very exciting goal. There are many protocols for immobilization of enzymes but very few are also very simple and/or very capable of improving enzyme properties. Novel immobilization protocols are still needed in order to achieve a massive implementation of enzymes as catalysts of the most complex chemical processes under the most benign experimental and environmental conditions. A critical review of enzyme immobilization under this point of view is still necessary.

Key Words

Enzymes and sustainable chemical industries enzyme immobilization protocols enzyme immobilization techniques enzyme properties 
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Copyright information

© Humana Press Inc. 2006

Authors and Affiliations

  • Jose M. Guisan
    • 1
  1. 1.Institute of Catalysis, CSIC, Campus UAM-CantoblancoMadridSpain

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