Isolation and Culture of Embryonic Pancreas and Liver

  • Zoë D. Burke
  • Wan-Chun Li
  • Jonathan M.W. Slack
  • David Tosh
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 633)

Abstract

Culturing embryonic tissue in an in vitro setting offers the unique ability to manipulate the external medium and therefore to investigate the pathways involved in regulating normal organogenesis as well as providing models for developmental disorders. Here we describe a system for the in vitro culture of the dorsal pancreatic buds and liver buds from mouse embryos. The tissues are dissected from day 9.0 or 11.5 mouse embryos. The tissues are placed on fibronectin-coated coverslips in serum-containing medium and allowed to attach. Over the next few days, the buds grow as flattened structures which are thin enough to allow the use of wholemount immunostaining methods.

Key words

Pancreas liver embryo culture 

Notes

Acknowledgements

The authors are grateful to the Wellcome Trust for funding this work.

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Copyright information

© Springer Science+Business Media, LLC 2010

Authors and Affiliations

  • Zoë D. Burke
    • 1
  • Wan-Chun Li
    • 1
  • Jonathan M.W. Slack
    • 1
    • 2
  • David Tosh
    • 1
  1. 1.Department of Biology and BiochemistryCentre for Regenerative Medicine, University of BathBathUK
  2. 2.Stem Cell Institute, University of MinnesotaMinneapolisUSA

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