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Imaging Ca2+ Signals in Xenopus Oocytes

  • Sheila L. Dargan
  • Angelo Demuro
  • Ian Parker
Part of the Methods in Molecular Biology™ book series (MIMB, volume 322)

Abstract

Xenopus oocytes have become a favored preparation in which to study the spatiotemporal dynamics of intracellular Ca2+ signaling. Advantages of the oocyte as a model cell system include its large size, lack of intracellular Ca2+ release channels other than the type 1 inositol trisphosphate receptor, and ease of expression of foreign receptors and channels. We describe the use of high-resolution fluorescence imaging techniques to visualize Ca2+ signals in Xenopus oocytes at levels ranging from global Ca2+ waves to single-channel Ca2+ microdomains.

Key Words

Ca2+ caged IP3 calcium confocal dye flash photolysis fluorescence imaging inositol IP3 linescan microinjection microscopy receptor signaling TIR video rate Xenopus oocytes 

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Copyright information

© Humana Press Inc., Totowa, NJ 2006

Authors and Affiliations

  • Sheila L. Dargan
    • 1
  • Angelo Demuro
    • 1
  • Ian Parker
    • 2
  1. 1.Department of Neurobiology and Behavior, McGaugh HallUniversity of California IrvineIrvine
  2. 2.Department of Neurobiology and BehaviorUniversity of California IrvineIrvine

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