Imaging Ca2+ Signals in Xenopus Oocytes
Xenopus oocytes have become a favored preparation in which to study the spatiotemporal dynamics of intracellular Ca2+ signaling. Advantages of the oocyte as a model cell system include its large size, lack of intracellular Ca2+ release channels other than the type 1 inositol trisphosphate receptor, and ease of expression of foreign receptors and channels. We describe the use of high-resolution fluorescence imaging techniques to visualize Ca2+ signals in Xenopus oocytes at levels ranging from global Ca2+ waves to single-channel Ca2+ microdomains.
Key WordsCa2+ caged IP3 calcium confocal dye flash photolysis fluorescence imaging inositol IP3 linescan microinjection microscopy receptor signaling TIR video rate Xenopus oocytes
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