Microtransplantation of Nigral Dopamine Neurons

A Step-by-Step Recipe
  • Guido Nikkhah
  • Christian Winkler
  • Alexandra Rödter
  • Madjid Samii
Part of the Neuromethods book series (NM, volume 36)


The cell suspension grafting procedure, as originally described by (1983), has become the standard protocol for the implantation of fetal neuronal cell suspensions to deep brain nuclei. Its major advantages, compared to the earlier studies of transplanting solid pieces of fetal tissue into cortical cavities overlying the caudate-putamen (CPU) or lateral ventricle (Stenevi et al., 1976; Björklund et al., 1979), have been the ability to graft to intraparenchymal target sites with less trauma and high stereotactic accuracy, and to manipulate the cells prior to implantation. It has become the standard technique for the preparation and implantation, not only of dopaminergic (DA-ergic), but also of noradrenergic, cholinergic, serotonergic, and γ-aminobutyric aid (GABA)-ergic graft tissue since then (Björklund and Dunnett, 1992; see also Dunnett and Björklund, this volume). For dopamine (DA)-rich transplants, usually the ventral mesencephalon (VM) of embryonic day 14–15 (E14–E15) rat fetuses are prepared by means of mechanical and enzymatic dissociation (Björklund et al., 1983; see also Barker and Dunnett, this volume). However, only 8–10% of these cells are DA neurons (Nikkhah et al., 1993b): The majority were GABA-ergic and other non-DA neuronal and glial precursor cells.


Graft Survival Glass Capillary Glial Fibrillary Acidic Protein Expression Ventral Mesencephalon Hamilton Microsyringe 
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Copyright information

© Humana Press Inc., Totowa, NJ 2000

Authors and Affiliations

  • Guido Nikkhah
    • 1
  • Christian Winkler
    • 1
  • Alexandra Rödter
    • 1
  • Madjid Samii
    • 1
  1. 1.Neurosurgical ClinicNordstadt HospitalHanoverGermany

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