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Sl Nuclease Analysis of RNA

  • Louis Lefebvre
  • Stéphane Viville
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The analysis of RNA molecules by S1 nuclease digestion constitutes an important tool for molecular studies of cellular processes, not only because of the historical significance of its contribution to messenger ribonucleic acids (mRNA) structure analyses, but also for its simplicity and wide range of applications Following a brief introduction to important aspects of RNA studies, this chapter presents a detailed description of the theory and principles underlying the technique of RNA mapping by S1 -nuclease digestion, as well as a discussion of the most relevant applications of this molecular technique

Keywords

Nuclease Digestion Protected Fragment Protected Band dsDNA Probe Mapping Analysis Feature 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc , Totowa, NJ 1998

Authors and Affiliations

  • Louis Lefebvre
    • 1
  • Stéphane Viville
    • 1
  1. 1.Wellcome/CRC InstituteCambrzdgeUK

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