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Extraction of Total RNA from Tissues and Cultured Cells

  • Sandeep Raha
  • Mingfu Ling
  • Frank Merante
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

The rsolatron of intact, high quality, total cellular RNA is often the starting point for many molecular biologrcal procedures (Fig. 1) There are numerous general methods for the rsolation of total cellular RNA (1, 2, 3, 4, 5, 6, 7, 8, 9). There are also many specialrzed methods for the rsolatron of RNA from specific tissues (8, 9, 10), various cell types (11), and subcellular organelles (7,12,13) In addmon, a number of methods describe the srmultaneous rsolatton of RNA and DNA (14, 15, 16, 17). Generally, the rationale for any tsolation procedure is to solubihze cellular components and simultaneously mactrvate mtracellular RNases while mamtaming brologrcally active RNA Therefore, the goal is to acquire purrfied cellular RNA in an intact form that can be a substrate for further mampulatrons, such as in vitro translation, RNase protectton, reverse transcrrptron, and Northern-blot analysis.

Keywords

RNase Activity Subcellular Organelle Polymerase Chain Reaction Procedure Subsequent Polymerase Chain Reaction Minimal Sample Handling 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc , Totowa, NJ 1998

Authors and Affiliations

  • Sandeep Raha
    • 1
  • Mingfu Ling
    • 1
  • Frank Merante
    • 1
  1. 1.Department of GeneticsHospital for Sick ChildrenCanada

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