Functionalization of Oligonucleotides by the Incorporation of Thio-Specific Reporter Groups

  • Jacqueline A Fidanza
  • Hiroaki Ozaki
  • Larry W. McLaughlin
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 26)

Abstract

The attachment of reporter groups, drug derivatives, or chemically reactive species to DNA in a sequence-specific manner has the potential to provide new materials for detailed spectroscopic and structural analyses as well as new classes of DNA therapeutics and diagnostics. Sequence specific labeling of a nucleic acid sequence can be achieved by a number of procedures that employ either the nucleobase, the carbohydrate or the phosphate residue as a site for attachment (for a recent review see ref. 1). Specific functional groups (such as terminal phosphomonoesters, see  Chapter 3) or the enhanced reactivity of selected sites on the purine or pyrimidine building blocks (such as the C5-position of pyrimidines, see  Chapter 2) are often used in order to covalently attach an appropriate linker or the desired reporter group. The manner in which the nucleic acid is labeled may be dictated by the specific study involved, but in general the principles of simplicity and versatility are best employed to guide the choice of labeling procedure.

Keywords

Trifluoroacetic Anhydride Phosphate Residue Reporter Group Triethylammonium Acetate Bacterial Alkaline Phosphatase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc., Totowa, NJHumana Press Inc 1994

Authors and Affiliations

  • Jacqueline A Fidanza
    • 1
  • Hiroaki Ozaki
    • 1
  • Larry W. McLaughlin
    • 1
  1. 1.Department of ChemistrBoston CollegeBoston

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