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Proteins are large molecules. A great many studies to investigate protein structure and function require that a protein be fragmented in order to produce pieces that are suitable for further detailed investigation. Some of the main types of experiments that require protein fragmentation are summarized in Section 1. of this chapter. The next two sections are concerned with the methods used to generate fragments of a protein; these can be grouped conveniently depending on whether the fragmentation causes denaturation or not. The final section of this chapter considers the use of recombinant protein technology to produce protein fragments.
KeywordsProteolytic Enzyme Peptide Bond Limited Proteolysis Triose Phosphate Isomerase Cyanogen Bromide
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