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Analytical Electrophoresis

  • Colin Simpson
Protocol
Part of the Biological Methods book series (BM)

Abstract

The first true use of electrophoresis for the separation of proteins developed from the pioneering work of Tiselius (1) in 1937, but the concepts and developments that led to the introduction of the technique occurred in the mid-nineteenth century with Faraday’s laws of electrolysis. These laws predated the first accepted demonstration of the chromatographic technique by about 50 years. From Faraday’s laws the concept of transport numbers was developed by Hittorf in 1850. In 1870, Kohlrausch demonstrated the measurement of conductivity using an ac Wheatstone bridge system with a telephone receiver as the null indicating device. From conductivities, the concept of ionic mobilities was developed. These three concepts are fundamental to the development of electrophoresis. The moving boundary method of Tiselius, however, although a superb separation method, was a very difficult technique to control and had the disadvantage that only the slowest and fastest migrating species could be obtained in pure form and then only in small quantities. It was therefore not suited for preparative use. Interestingly, it was Martin’s development of partition and paper chromatography (2,3) that gave the stimulus to develop electrophoretic methods capable of separating components into zones using materials similar to those used in chromatography, Development was rapid, and there is now a powerful armoury of methods suitable for protein separation.

Keywords

Protein Separation Focus Zone Separate Zone Beryllium Oxide Carrier Ampholyte 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc. 1988

Authors and Affiliations

  • Colin Simpson
    • 1
  1. 1.Department of Chemistry, Chelsea CollegeUniverszty of LondonLondonUK

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