Polypeptide Sequence Analysis Using Gas Chromatography-Mass Spectrometry
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Polypeptide sequence analyses have been carried out using a variety of chemical and enzymatic technologies. Although the most common is the Edman procedure, in many cases methodologies involving gas chromatography-mass spectrometry (GC-MS) may be the preferred route for these structure determinations. For example, a blocked polypeptide N-terminus is not a barrier to structure determinations as it is in the Edman technique. Also, Ser and Thr residues are always identifiable by GC-MS technologies, and C-terminal sequence determinations are as readily accessible as N-terminal analyses when GC-MS technologies are employed. In addition, unusual amino acid residues and posttranslational modifications to the polypeptide can be determined by GC-MS methodologies for sequence analysis.