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Microsequence Analysis by Enzymatic Methods

  • Barry Nelson Jones
Protocol
Part of the Biological Methods book series (BM)

Abstract

The amino-terminal sequence analysis of polypeptides by the Edman procedure has become a standard and routine method for the protein chemist. The procedure combines the specificity of end-group analysis with the advantages of a cyclic stepwise process. This powerful technique has been automated and normally permits the unambiguous identification of the first 30–50 residues of a polypeptide. Recent modifications of the automated Edman procedure now permit the microsequence analysis of proteins (see chapter by Hunkapiller in this volume). This important development makes possible the structure analysis of proteins at the picomole level. Thus, many protein structural studies are now being undertaken that would have been impossible a few years ago.

Keywords

Amino Acid Analysis Lyophilize Powder Release Amino Acid Amino Acid Amide Cyanogen Bromide Fragment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© The Humana Press Inc. 1986

Authors and Affiliations

  • Barry Nelson Jones
    • 1
  1. 1.Unigene Laboratories, Inc.Fairfield

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