Carbohydrate Biotechnology Protocols pp 235-254
Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides
The realization that the oligosaccharide moieties of glycoconjugates, such as glycoproteins and glycolipids, are involved in important intra- and intercellular of important oligosaccharide structures as tools in analytical and therapeutic studies (seerefs.1 and 2 for reviews). A number of efficient chemical procedures have been developed for the synthesis of the “glyco-” part (seerefs.3, 4, 5, 6, 7 for review). However, for the synthesis of a given saccharide structure, an individual strategy has to be set up comprising many laborious protection and deprotection steps for stereo-controlled synthesis, which finally results in only moderate overall yields. As an example, the synthesis of N-acetyllactosamine (LacNAc) involves 12 steps and needs 3 months of lab work (7). A scale-up of the chemical procedure often encounters environmental problems because all steps are carried out in organic solvents.