Carbohydrate Biotechnology Protocols pp 235-254

Part of the Methods in Biotechnology™ book series (MIBT, volume 10)

Application of Sucrose Synthase in the Synthesis of Nucleotide Sugars and Saccharides

  • Astrid Zervosen
  • Lothar Elling
Protocol

Abstract

The realization that the oligosaccharide moieties of glycoconjugates, such as glycoproteins and glycolipids, are involved in important intra- and intercellular of important oligosaccharide structures as tools in analytical and therapeutic studies (seerefs.1 and 2 for reviews). A number of efficient chemical procedures have been developed for the synthesis of the “glyco-” part (seerefs.3, 4, 5, 6, 7 for review). However, for the synthesis of a given saccharide structure, an individual strategy has to be set up comprising many laborious protection and deprotection steps for stereo-controlled synthesis, which finally results in only moderate overall yields. As an example, the synthesis of N-acetyllactosamine (LacNAc) involves 12 steps and needs 3 months of lab work (7). A scale-up of the chemical procedure often encounters environmental problems because all steps are carried out in organic solvents.

Copyright information

© Humana Press Inc., Totowa, NJ 1999

Authors and Affiliations

  • Astrid Zervosen
    • 1
  • Lothar Elling
    • 1
  1. 1.Institute of Enzyme TechnologyHeinrich-Heine-University Düsseldorf Research Center JülichJülichGermany

Personalised recommendations