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In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei

  • Mark A. Sanders
  • David M. Gartner
Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Ultrastructural studies usually require that the sample be sacrificed and the tissue of interest be prepared by specialized fixation procedures. The goal of any given ultrastructural study is to observe and record the distribution and organization of a cellular constituent(s), in a way that most closely represents its in vivo distribution. Chemical fixations with aldehydes or cold solvents are often employed to preserve specimens for ultrastructural studies. However, these studies are only “snapshots” in time of the chemical termination of the sample. There can often be difficulties in interpreting the results, because of the harsh manipulations that chemical fixations can induce upon the specimen. Another practical disadvantage of chemical fixation in the preparation of biological specimens for microscopy is the time required to complete the process.

Keywords

Root Meristem Grape Juice Cold Spot Drosophila Embryo Chemical Fixation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Humana Press Inc., Totowa, NJ 2001

Authors and Affiliations

  • Mark A. Sanders
    • 1
  • David M. Gartner
    • 2
  1. 1.Imaging Center, College of Biological SciencesUniversity of MinnesotaSt. Paul
  2. 2.University of MinnesotaSt. Paul

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