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2 Microbial Cell Disruption by High-Pressure Homogenization

  • Anton P. J. Middelberg
Protocol
Part of the Methods in Biotechnology book series (MIBT, volume 9)

Abstract

The disruption of a cell’s wall is often a primary step in product isolation, particularly when hosts such as Escherichia coli and Saccharomyces cerevisiae, which generally do not excrete product, are employed. Of the available methods, high-pressure homogenization is dominant at moderate or large process volumes.

Keywords

Cell Disruption Photon Correlation Spectroscopy Disruption Efficiency Tank Level Electrical Sense Zone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Humana Press Inc. 2000

Authors and Affiliations

  • Anton P. J. Middelberg
    • 1
  1. 1.Department of Chemical EngineeringUniversity of CambridgeCambridgeUK

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