Acquisition of Mass Spectrometry Data of Carotenoids: A Focus on Big Data Management

  • Antonio Pérez-Gálvez
  • Isabel Viera
  • María RocaEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2083)


Accurate determination of carotenoid profile in plant tissues and food samples requires the application of hyphenated analytical resources including HPLC with high-resolution hybrid mass spectrometers. The high analytical power of modern MS equipment means the generation of Big Data resulting from the independent and complementary physicochemical properties of the target compounds that requires a complex processing to unravel the results. The present protocol describes a complete pipeline methodology for high-throughput analysis of carotenoids based on mass spectrometry (MS). After an exhaustive extraction, the workflow includes the acquisition of HPLC-hr-MS and MS2 spectra assisted step by step by specific post-processing software.

Key words

Carotenoids Mass spectrometry Product ions Exact mass Isotopic pattern HPLC-MS qQ-TOF APCI Tandem mass spectra Software 



This work was supported by the Ministerio de Ciencia, Investigación y Universidades, Agencia Estatal de Investigación y Fondo Europeo de Desarrollo Regional (FEDER), project numbers RTI2018-095415-B-I00 and AGL2017-87884-R. Antonio Pérez-Gálvez is a tenured scientist at the Spanish National Research Council (CSIC).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Antonio Pérez-Gálvez
    • 1
  • Isabel Viera
    • 1
  • María Roca
    • 1
    Email author
  1. 1.Food Phytochemistry DepartmentInstituto de la Grasa (CSIC)SevillaSpain

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