In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

  • José A. Guijarro
  • Jessica Méndez
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)


Bioluminescent reporters and advanced luciferase technologies are useful to study host–pathogen interactions. This chapter describes the use of the luxCDABE operon from Photorhabdus luminescens as a tool to analyze the progression of the fish pathogen Yersinia ruckeri during the infection of rainbow trout, as well as the quantification of promoter activity of specific bacterial genes during host colonization.

Key words

Yersinia ruckeri Rainbow trout Infection process Bioluminescence imaging luxCDABE operon IVIS® Imaging System Promoter activity 


  1. 1.
    Waidmann MS, Bleichrodt FS, Laslo T, Riedel CU (2011) Bacterial luciferase reporters: the Swiss army knife of molecular biology. Bioeng Bugs 2:8–16. Scholar
  2. 2.
    Contag CH, Contag PR, Mullins JI, Spilman SD, Stevenson DK, Benaron DA (1995) Photonic detection of bacterial pathogen in living host. Mol Microbiol 18:593–603. Scholar
  3. 3.
    Francis KP, Joh D, Bellinger-Kawahara C, Hawkinson MJ, Purchio TF, Contag PR (2000) Monitoring bioluminescent Staphylococcus aureus infections in living mice using a novel luxABCDE construct. Infect Immun 68:3594–35600CrossRefGoogle Scholar
  4. 4.
    Francis KP, Yu J, Bellinger-Kawahara C, Joh D, Hawkinson MJ et al (2001) Visualizing pneumococcal infections in the lungs of live mice using bioluminescence Streptococcus pneumoniae transformed with a novel Gram-positive lux transposon. Infect Immun 69:3350–3358. Scholar
  5. 5.
    Meighen EA, Szittner RB (1992) Multiple repetitive elements and organization of the lux operons of luminescent terrestrial bacteria. J Bacteriol 174:5371–5381. Scholar
  6. 6.
    Fernandez L, Mendez J, Guijarro JA (2007) Molecular virulence mechanisms of the fish pathogen Yersinia ruckeri. Vet Microbiol 125:1–10. Scholar
  7. 7.
    Guijarro JA, Garcia-Torrico AI, Cascales D, Mendez J (2018) The infection process of Yersinia ruckeri: reviewing the pieces of the jigsaw puzzle. Front Cell Infect Microbiol 8:218. Scholar
  8. 8.
    Mendez J, Guijarro JA (2012) In vivo monitoring of Yersinia ruckeri in fish tissues: progression and virulence gene expression. Environ Microbiol Rep 5:179–185. Scholar
  9. 9.
    Romalde JL, Conchas RF, Toranzo AE (1991) Evidence that Yersinia ruckeri possesses a high affinity iron uptake system. FEMS Microbiol Lett 80:121–126. Scholar
  10. 10.
    Simon R, Priefer U, Pühler A (1983) A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Nat Biotechnol 1:784–791. Scholar
  11. 11.
    Bjarnason J, Southward CM, Surette MG (2003) Genomic profiling of iron-responsive genes in Salmonella enterica serovar Typhimurium by high-throughput screening of a random promoter library. J Bacteriol 185:4973–4982. Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • José A. Guijarro
    • 1
  • Jessica Méndez
    • 1
  1. 1.Departamento de Biología Funcional, Facultad de Medicina, IUBAUniversidad de OviedoOviedoSpain

Personalised recommendations