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In Vivo Bioluminescent Imaging of Yersinia ruckeri Pathogenesis in Fish

  • José A. Guijarro
  • Jessica Méndez
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)

Abstract

Bioluminescent reporters and advanced luciferase technologies are useful to study host–pathogen interactions. This chapter describes the use of the luxCDABE operon from Photorhabdus luminescens as a tool to analyze the progression of the fish pathogen Yersinia ruckeri during the infection of rainbow trout, as well as the quantification of promoter activity of specific bacterial genes during host colonization.

Key words

Yersinia ruckeri Rainbow trout Infection process Bioluminescence imaging luxCDABE operon IVIS® Imaging System Promoter activity 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • José A. Guijarro
    • 1
  • Jessica Méndez
    • 1
  1. 1.Departamento de Biología Funcional, Facultad de Medicina, IUBAUniversidad de OviedoOviedoSpain

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