Advertisement

Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells

  • Setsuko Tsuboi
  • Takashi JinEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)

Abstract

Detection of apoptotic cells is crucial for understanding the mechanism of diseases and for therapy development. So far, visible-emitting fluorescent probes such as FITC-labeled Annexin V has been widely used for the detection of apoptotic cells. However, such probes cannot be applied to noninvasive imaging in the near-infrared (NIR) region. Compared with visible light, NIR light is highly permeable in turbid biological samples and tissues. In addition, NIR optical imaging has several advantages such as lower autofluorescence and scattering from biological samples, leading to clearer images with high signal to background ratios. Here, we describe the synthesis and application of bioluminescence resonance energy transfer (BRET)-coupled quantum dots (QDs) for the NIR optical imaging of apoptotic cells.

Key words

Bioluminescence resonance energy transfer (BRET) Renilla luciferase Near-infrared imaging (NIR imaging) Quantum dots (QDs) Annexin V Apoptotic cell 

References

  1. 1.
    Taylor RC, Cullen SP, Martin SJ (2008) Apoptosis: controlled demolition at the cellular level. Nat Rev Mol Cell Biol 9:231–241CrossRefGoogle Scholar
  2. 2.
    Fuchs Y, Steller H (2015) Live to die another way: modes of programmed cell death and the signals emanating from dying cells. Nat Rev Mol Cell Biol 16:329–344CrossRefGoogle Scholar
  3. 3.
    Elmore S (2007) Apoptosis: a review of programmed cell death. Toxicol Pathol 35:495–516CrossRefGoogle Scholar
  4. 4.
    Fuchs Y, Steller H (2011) Programmed cell death in animal development and disease. Cell 147:742–758CrossRefGoogle Scholar
  5. 5.
    van Genderen HO, Kenis H, Hofstra L, Narula J, Reutelingsperger CP (2008) Extracellular annexin A5: functions of phosphatidylserine-binding and two-dimensional crystallization. Biochim Biophys Acta 1783:953–963Google Scholar
  6. 6.
    Lizarbe MA, Barrasa JI, Olmo N, Gavilances F, Turnay J (2013) Annexin-phospholipid interactions. Functional implications. Int J Mol Sci 14:2652–2683CrossRefGoogle Scholar
  7. 7.
    Nazari M, Minai-Tehrai A, Emamzadeh R (2014) Comparison of different probes based on labeled annexin V for detection of apoptosis. RSC Adv 4:45128–45135CrossRefGoogle Scholar
  8. 8.
    Koopman G, Reutelingsperger C, Kuijten G, Keehnen R, Pals S, Van Oers M (1994) Annexin V for flow cytometric detection of phosphatidylserine expression on B cells undergoing apoptosis. Blood 84:1415–1420CrossRefGoogle Scholar
  9. 9.
    Petrovsky A, Schellenberger E, Josephson L, Weissleder R, Bogdanov A Jr (2003) Near-infrared fluorescent imaging of tumor apoptosis. Cancer Res 63:1936–1942PubMedGoogle Scholar
  10. 10.
    Hasegawa M, Tsukasaki Y, Ohyanagi T, Jin T (2013) Bioluminescence resonance energy transfer coupled near-infrared quantum dots using GST-tagged luciferase for in vivo imaging. Chem Commun 49:228–230CrossRefGoogle Scholar
  11. 11.
    Samanta A, Walper SA, Susumu K, Dwyer CL, Medinz IL (2015) An enzymatically-sensitized sequential and concentric energy transfer relay self-assembled around semiconductor quantum dots. Nanoscale 7:7603–7614CrossRefGoogle Scholar
  12. 12.
    Yu X, Wen K, Wang Z, Zhang X, Li C, Zhnag S, Shen J (2016) General bioluminescence resonance energy transfer homogeneous immunoassay for small molecules based on quantum dots. Anal Chem 88:3512–3520CrossRefGoogle Scholar
  13. 13.
    Kamkaew A, Sun H, England CG, Cheng L, Liu Z, Cai W (2016) Quantum dot–NanoLuc bioluminescence resonance energy transfer enables tumor imaging and lymph node mapping in vivo. Chem Commun 52:6997–7000CrossRefGoogle Scholar
  14. 14.
    Weissleder R (2001) A clearer vision for in vivo imaging. Nat Biotechnol 19:316–317CrossRefGoogle Scholar
  15. 15.
    Srikantha T, Klapach A, Lorenz WW, Tsai LK, Laughlin LA, Gorman JA, Soll DR (1996) The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans. J Bacteriol 178:121–129CrossRefGoogle Scholar
  16. 16.
    Jin T, Yoshioka Y, Fujii F, Komai Y, Seki J, Seiyama A (2008) Gd3+-functionalized near-infrared quantum dots for in vivo dual modal (fluorescence/magnetic resonance) imaging. Chem Commun 44:5764–5766CrossRefGoogle Scholar
  17. 17.
    Jin T, Fujii F, Komai Y, Seki J, Seiyama A, Yoshioka Y (2008) Preparation and characterization of highly fluorescent, glutathione-coated near infrared quantum dots for in vivo fluorescence imaging. Int J Mol Sci 9:2044–2061CrossRefGoogle Scholar
  18. 18.
    Medintz IL, Uyeda HT, Goldman ER, Mattoussi H (2005) Quantum dot bioconjugates for imaging, labelling and sensing. Nat Mater 4:435–446CrossRefGoogle Scholar
  19. 19.
    Lao UL, Mulchandani A, Chen W (2006) Simple conjugation and purification of quantum dot-antibody complexes using a thermally responsive elastin-protein L scaffold as immunofluorescent agents. J Am Chem Soc 128:14756–14757CrossRefGoogle Scholar
  20. 20.
    Alam R, Karam LM, Doane TL, Zylstra J, Fontaine DM, Branchini BR, Maye MM (2014) Near infrared bioluminescence resonance energy transfer from firefly luciferase-quantum dot bionanoconjugates. Nanotechnology 25:495606. (7pp)CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Riken Center for Biosystems Dynamics ResearchRikenJapan

Personalised recommendations