Bioluminescence Resonance Energy Transfer (BRET) Coupled Near-Infrared Imaging of Apoptotic Cells

  • Setsuko Tsuboi
  • Takashi JinEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)


Detection of apoptotic cells is crucial for understanding the mechanism of diseases and for therapy development. So far, visible-emitting fluorescent probes such as FITC-labeled Annexin V has been widely used for the detection of apoptotic cells. However, such probes cannot be applied to noninvasive imaging in the near-infrared (NIR) region. Compared with visible light, NIR light is highly permeable in turbid biological samples and tissues. In addition, NIR optical imaging has several advantages such as lower autofluorescence and scattering from biological samples, leading to clearer images with high signal to background ratios. Here, we describe the synthesis and application of bioluminescence resonance energy transfer (BRET)-coupled quantum dots (QDs) for the NIR optical imaging of apoptotic cells.

Key words

Bioluminescence resonance energy transfer (BRET) Renilla luciferase Near-infrared imaging (NIR imaging) Quantum dots (QDs) Annexin V Apoptotic cell 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Riken Center for Biosystems Dynamics ResearchRikenJapan

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