High-Throughput Bioluminescence Imaging and Reporter Gene Assay with 3D Spheroids from Human Cell Lines

  • Maria Maddalena Calabretta
  • Laura Montali
  • Antonia Lopreside
  • Elisa MicheliniEmail author
  • Aldo Roda
Part of the Methods in Molecular Biology book series (MIMB, volume 2081)


3D cell culture models represent an attractive approach to decode intracellular and intercellular signaling, providing biologically relevant information and predictive data. Bioluminescent reporter gene assays and bioluminescence imaging in 3D cell models are very promising bioanalytical tools for several applications.

Here we report a very straightforward method for bioluminescence imaging and bioluminescent reporter gene assays in 3D cell-culture models. Both the assays can be easily implemented in laboratories equipped with basic cell culture facilities and instrumentation for bioluminescence detection, that is, low-light detectors connected to inverted microscopes and luminometers, without the need for additional equipment.

Key words

Bioluminescence Spheroids Imaging Reporter gene 3D cell models Luciferase 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Maria Maddalena Calabretta
    • 1
  • Laura Montali
    • 1
  • Antonia Lopreside
    • 1
  • Elisa Michelini
    • 1
    • 2
    • 3
    Email author
  • Aldo Roda
    • 1
    • 2
  1. 1.Department of Chemistry “Giacomo Ciamician”University of BolognaBolognaItaly
  2. 2.INBB, Istituto Nazionale di Biostrutture e BiosistemiRomeItaly
  3. 3.Health Sciences and Technologies-Interdepartmental Center for Industrial Research (HST-ICIR)University of BolognaBolognaItaly

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