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Staining MIF in Cells for Confocal Microscopy

  • James HarrisEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

Confocal microscopy is a powerful technique for immunofluorescence imaging of cells and tissues. The technique allows for detailed analysis of intracellular localization of molecules, as well as three-dimensional representation and analysis of samples, and can be used as a gateway to more advanced techniques, including FLIM-FRET and super-resolution microscopy. Relatively few studies have used confocal microscopy to study intracellular localization of macrophage migration inhibitory factor (MIF) in detail. This chapter outlines basic protocols and tips for staining MIF in fixed cells for confocal analysis.

Key words

Antibody staining Confocal Fluorophores Immunofluorescence 

References

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    Harris J et al (2019) Rediscovering MIF: new tricks for an old cytokine. Trends Immunol.  https://doi.org/10.1016/j.it.2019.03.002CrossRefGoogle Scholar
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    Lang T et al (2018) Macrophage migration inhibitory factor is required for NLRP3 inflammasome activation. Nat Commun 9(1):2223CrossRefGoogle Scholar
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    Merk M et al (2009) The Golgi-associated protein p115 mediates the secretion of macrophage migration inhibitory factor. J Immunol 182(11):6896–6906CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical CentreMonash UniversityClaytonAustralia

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