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Flow Cytometry Phenotyping of Bone Marrow-Derived Macrophages from Wild-Type and Mif−/− Mice

  • Jacqueline K. FlynnEmail author
  • Nadia S. Deen
  • James Harris
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

Phenotyping cells by flow cytometry is a powerful way to identify cell type and any morphological changes during cell culture. The staining procedure used in this chapter enables the characterization of mouse macrophages by a flow cytometry antibody panel which can be used for both bone marrow-derived macrophages (BMM) and macrophages derived from other tissues, such as the mouse spleen or peritoneal cavity. The surface and intracellular staining methods are versatile and can be applied to flow cytometry staining of several different cell types by changing the surface markers used with knowledge of which receptors are expressed on different cell types.

Key words

BMM Macrophages Fluorochromes Flow cytometry Cell surface receptors MIF 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Jacqueline K. Flynn
    • 1
    Email author
  • Nadia S. Deen
    • 2
  • James Harris
    • 1
  1. 1.Rheumatology Group, Centre for Inflammatory Diseases, Department of Medicine, School of Clinical Sciences at Monash Health, Faculty of Medicine, Nursing and Health Sciences, Monash Medical CentreMonash UniversityClaytonAustralia
  2. 2.The Ritchie Centre, Hudson Institute of Medical ResearchMonash UniversityClaytonAustralia

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