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Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells

  • Tali LangEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.

Key words

MIF BMM BMDC Cell culture ELISA Western blot analysis 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.The Szalmuk Family Department of Medical Oncology, Level 2Cabrini InstituteMalvernAustralia

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