Analysis of Secreted MIF from Cultured Murine Bone Marrow-Derived Immune Cells

  • Tali LangEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)


Macrophage migration inhibitory factor (MIF) is expressed and released ubiquitously by numerous cell types and tissues. MIF is detected and constitutively expressed at the protein level both intra- and extracellularly. This chapter outlines methods for cultivating, purifying, detecting, and quantifying concentrations of MIF from murine primary derived macrophages and dendritic cell culture supernatants.

Key words

MIF BMM BMDC Cell culture ELISA Western blot analysis 


  1. 1.
    Lang T et al (2015) MIF: implications in the pathoetiology of systemic lupus erythematosus. Front Immunol 6:577CrossRefGoogle Scholar
  2. 2.
    Bloom BR, Bennett B (1966) Mechanism of a reaction in vitro associated with delayed-type hypersensitivity. Science 153(3731):80–82CrossRefGoogle Scholar
  3. 3.
    David JR (1966) Delayed hypersensitivity in vitro: its mediation by cell-free substances formed by lymphoid cell-antigen interaction. Proc Natl Acad Sci U S A 56(1):72–77CrossRefGoogle Scholar
  4. 4.
    Baugh JA, Bucala R (2002) Macrophage migration inhibitory factor. Crit Care Med 30(1 Supp):S27–S35CrossRefGoogle Scholar
  5. 5.
    Calandra T, Roger T (2003) Macrophage migration inhibitory factor: a regulator of innate immunity. Nat Rev Immunol 3(10):791–800CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.The Szalmuk Family Department of Medical Oncology, Level 2Cabrini InstituteMalvernAustralia

Personalised recommendations