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The Effect of Macrophage Migration Inhibitory Factor on Intestinal Permeability: FITC-Dextran Serum Measurement and Transmission Electron Microscopy

  • Milica Vujičić
  • Sanja Despotović
  • Tamara Saksida
  • Ivana StojanovićEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

Macrophage migration inhibitory factor (MIF) is a molecule with multiple functions: from enforcing the immune system to fight bacterial infection to the regulation of insulin activity. Also, MIF is expressed by enterocytes that line the intestinal border toward the lumen, and in M cells, where it regulates phagocytosis of antigens from the lumen of the gut and their transport to Peyer’s patches. Since there were no data on the role of MIF in the maintenance of the intestinal barrier, we used MIF-deficient mice bred on C57BL/6 background as a model for the investigation of intestinal permeability. The obtained results indicate that the absence of MIF increases intestinal permeability. Here we describe two methods for measuring intestinal permeability in mice: detection of orally delivered FITC-dextran in the serum and transmission electron microscopy used for visualization and measurement of cell-to-cell connections width.

Key words

Macrophage migration inhibitory factor Intestinal permeability FITC-dextran Transmission electron microscopy 

Notes

Acknowledgements

Supported by the Ministry of Education, Science and Technological Development of the Republic of Serbia (grant 173013 and 175005).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Milica Vujičić
    • 1
  • Sanja Despotović
    • 1
  • Tamara Saksida
    • 1
  • Ivana Stojanović
    • 2
    Email author
  1. 1.Institute for Biological Research “Sinisa Stankovic”University of BelgradeBeogradSerbia
  2. 2.Faculty of MedicineUniversity of Belgrade, Institute of Histology and EmbryologyBeogradSerbia

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