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Methods to Determine the Effects of MIF on In Vitro Osteoclastogenesis Using Murine Bone Marrow-Derived Cells and Human Peripheral Blood Mononuclear Cells

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2080)

Abstract

Osteoclasts are the only cells that are capable of resorbing bones, and they are involved in multiple diseases and disorders. This chapter will describe several in vitro osteoclastogenesis methods, which allows further investigation of molecular mechanisms of osteoclastogenesis in normal physiological and disease conditions. This chapter includes a protocol for isolating osteoclast progenitors from mouse bone marrow and human peripheral blood, as well as obtaining murine osteoblasts for the coculture system. Furthermore, culture and identification of multinucleated osteoclasts in vitro is also described in this chapter.

Key words

Osteoclast Osteoclastogenesis MIF RANKL M-CSF TRAP Actin ring Bone resorption 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Biochemistry and Molecular Medicine, School of MedicineUniversity of California at DavisSacramentoUSA
  2. 2.Institute for Pediatric Regenerative Medicine, Shriners Hospitals for Children-Northern California, UC Davis School of MedicineSacramentoUSA

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