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Assessing ADC Plasma Stability by LC-MS Methods

  • Cong Wei
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2078)

Abstract

Plasma stability of ADCs can have a profound impact on ADC efficacy and safety. LC-MS methods enable the detection and characterization of ADC to evaluate its stability in plasma. Here we describe a procedure and LC-MS method for assessing ADC plasma stability.

Key words

Antibody–drug conjugate (ADC) Immunoaffinity capture LC-MS Drug-to-antibody ratio (DAR) Anti-human Fc Ab Biotinylation 

References

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    Shen BQ, Xu K, Liu L et al (2012) Conjugation site modulates the in vivo stability and therapeutic activity of antibody-drug conjugates. Nat Biotechnol 30(2):184–189CrossRefGoogle Scholar
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    Grafmuller L, Wei C, Ramanathan R et al (2016) Unconjugated payload quantification and DAR characterization of antibody-drug conjugates using high-resolution MS. Bioanalysis 8(16):1663–1678CrossRefGoogle Scholar
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    Wei C, Zhang G, Clark T et al (2016) Where did the linker-payload go? A quantitative investigation on the destination of the released linker-payload from an antibody-drug conjugate with a Maleimide linker in plasma. Anal Chem 88(9):4979–4986CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Cong Wei
    • 1
  1. 1.Drug Metabolism and PharmacokineticsBiogen Inc.CambridgeUSA

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