Assessing ADC Plasma Stability by LC-MS Methods
Plasma stability of ADCs can have a profound impact on ADC efficacy and safety. LC-MS methods enable the detection and characterization of ADC to evaluate its stability in plasma. Here we describe a procedure and LC-MS method for assessing ADC plasma stability.
Key wordsAntibody–drug conjugate (ADC) Immunoaffinity capture LC-MS Drug-to-antibody ratio (DAR) Anti-human Fc Ab Biotinylation