Assessing ADC Plasma Stability by LC-MS Methods

  • Cong Wei
Part of the Methods in Molecular Biology book series (MIMB, volume 2078)


Plasma stability of ADCs can have a profound impact on ADC efficacy and safety. LC-MS methods enable the detection and characterization of ADC to evaluate its stability in plasma. Here we describe a procedure and LC-MS method for assessing ADC plasma stability.

Key words

Antibody–drug conjugate (ADC) Immunoaffinity capture LC-MS Drug-to-antibody ratio (DAR) Anti-human Fc Ab Biotinylation 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Cong Wei
    • 1
  1. 1.Drug Metabolism and PharmacokineticsBiogen Inc.CambridgeUSA

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