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Characterization of ADCs by Capillary Electrophoresis

  • Wenjing Ning
  • Yanqun ZhaoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2078)

Abstract

Capillary electrophoresis (CE) is a highly efficient separation technique that resolves ions based on their electrophoretic mobility in the presence of an applied voltage. It has been broadly applied for characterizing biotherapeutics including ADCs. In this chapter, step-by-step procedures for characterizing ADCs using CE will be described with focus placed on reduced and non-reduced capillary electrophoresis sodium dodecyl sulfate (CE-SDS) for purity determination and imaged capillary isoelectric focusing (iCIEF) for charge heterogeneity analysis.

Key words

Antibody-drug conjugates CE Reduced and non-reduced CE-SDS iCIEF Charge heterogeneity 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Process Analytical ChemistryAbbVie Inc.North ChicagoUSA

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