Chimeric RNA pp 95-107 | Cite as
Detection of Group II Intron-Generated Chimeric mRNAs in Bacterial Cells
Protocol
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Abstract
Chimeric RNAs are the transcripts composed of exons from two separate genes or transcripts. Although the presence of these joined RNA molecules have mainly been documented in a variety of eukaryotes, we recently demonstrated that the Ll.LtrB group II intron, from the gram-positive bacterium Lactococcus lactis, can generate chimeric mRNAs through a novel intergenic trans-splicing pathway. Here we describe the detailed experimental procedures to detect group II intron-generated mRNA–mRNA chimeras from total RNA extracts using stringent reverse transcription conditions along with a reverse splicing-deficient group II intron as a negative control.
Key words
Group II intron Ll.LtrB Bacteria Lactococcus lactis Trans-splicing Chimeric mRNA Circular RNA CircularizationReferences
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