Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent

  • Edward T. Wargent
Part of the Methods in Molecular Biology book series (MIMB, volume 2076)


Under in vivo conditions, the study of physiological and pharmacological functions of an organ is difficult due to whole-body interactions with the organ. Thus, an in vitro technique for the perfusion of isolated pancreata was developed for physiologic and response studies including the investigation of endocrine function and secretory responsiveness under a variety of diabetes-associated conditions. The pancreas is isolated from the connecting spleen, stomach, and duodenum and transferred to a pre-warmed chamber where it is perfused in isolation from all other organs. A detailed description of the isolation of pancreata from rats and mice and the perfusion apparatus is described, as well as the measurement of glucose-stimulated insulin secretion using an in-house-developed radioimmunoassay.

Key words

Pancreas Perfusion Insulin Radioimmunoassay 


  1. 1.
    Sussman KE, Vaughan GD, Timmer RF (1966) An in vitro method for studying insulin secretion in the perfused isolated rat pancreas. Metabolism 15:466–476CrossRefGoogle Scholar
  2. 2.
    Emilsson V, Liu YL, Cawthorne MA, Morton NM, Davenport M (1997) Expression of the functional leptin receptor mRNA in pancreatic islets and direct inhibitory action of leptin on insulin secretion. Diabetes 46:313–316CrossRefGoogle Scholar
  3. 3.
    de Heer J, Rasmussen C, Coy DH, Holst JJ (2008) Glucagon-like peptide-1, but not glucose-dependent insulinotropic peptide, inhibits glucagon secretion via somatostatin (receptor subtype 2) in the perfused rat pancreas. Diabetologia 51:2263–2270CrossRefGoogle Scholar
  4. 4.
    Göncz E, Strowski MZ, Grötzinger C, Nowak KW, Kaczmarek P, Sassek M, Mergler S, El-Zayat BF, Theodoropoulou M, Stalla GK, Wiedenmann B, Plöckinger U (2008) Orexin-A inhibits glucagon secretion and gene expression through a Foxo1-dependent pathway. Endocrinology 149:1618–1626CrossRefGoogle Scholar
  5. 5.
    Egido EM, Hernández R, Marco J, Silvestre RA (2008) Effect of obestatin on insulin, glucagon and somatostatin secretion in the perfused rat pancreas. Regul Pept 152:61–66CrossRefGoogle Scholar
  6. 6.
    Tseng C-C, Zhang X-Y, Wolfe MM (1999) Effect of GIP and GLP-1 antagonists on insulin release in the rat. Am J Physiol Endocrinol Metab 276:E1049–E1054CrossRefGoogle Scholar
  7. 7.
    Eisenman JR, Chew BP (1983) Polyethylene Glycol in conjunction with a secondary antibody for 24 hour radioimmunoassay of bovine prolactin and growth hormone. J Dairy Science 66:1174–1179CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Edward T. Wargent
    • 1
  1. 1.Buckingham Institute of Translational MedicineUniversity of BuckinghamBuckinghamUK

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