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Measurement of Insulin Secretion Using Pancreas Perfusion in the Rodent

  • Edward T. Wargent
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2076)

Abstract

Under in vivo conditions, the study of physiological and pharmacological functions of an organ is difficult due to whole-body interactions with the organ. Thus, an in vitro technique for the perfusion of isolated pancreata was developed for physiologic and response studies including the investigation of endocrine function and secretory responsiveness under a variety of diabetes-associated conditions. The pancreas is isolated from the connecting spleen, stomach, and duodenum and transferred to a pre-warmed chamber where it is perfused in isolation from all other organs. A detailed description of the isolation of pancreata from rats and mice and the perfusion apparatus is described, as well as the measurement of glucose-stimulated insulin secretion using an in-house-developed radioimmunoassay.

Key words

Pancreas Perfusion Insulin Radioimmunoassay 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Edward T. Wargent
    • 1
  1. 1.Buckingham Institute of Translational MedicineUniversity of BuckinghamBuckinghamUK

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