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Assessing Islet Transplantation Outcome in Mice

  • Aileen J. F. KingEmail author
  • Chloe L. Rackham
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2076)

Abstract

Islet transplantation is a potential treatment for Type 1 diabetes; however, improvements need to be made before it could become clinically widely available. In preclinical studies, the mouse is often used to model islet transplantation, with most studies aiming to improve transplantation outcome by manipulating the islets prior to transplantation or by treating the recipient mouse. Here, we describe the process of islet transplantation in the mouse, including how one can make the mouse diabetic, isolate donor islets, and transplant the islets into two different sites. Finally, we discuss how to assess the outcome of the transplantation in order to determine whether the experimental intervention has been beneficial.

Key words

Islet transplantation Mouse Islet isolation Intraportal islet transplantation Renal subcapsular islet transplantation 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Diabetes Research Group, School of Life Course SciencesKing’s College LondonLondonUK

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