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Quantification of Pancreatic Islets: Using Image Analysis Tools

  • Parvathy E. HarikumarEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2076)

Abstract

Histological image analysis is becoming an increasingly important tool for research in biological science. They are important in analyzing biological systems on various scales, from structural details to determination of number of cells, its area, localization, and concentration. This chapter focuses on analysis of pancreatic sections stained for insulin and glucagon using a commercially available software.

Key words

Histology Pancreas Immunohistochemistry Whole-slide image Image analysis VisiomorphDP ImageJ 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.National Institute for Biological Standards and Control (NIBSC) and Buckingham Institute of Translational Medicine, University of BuckinghamBuckinghamUK

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