Native Protein Mass Spectrometry
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In native mass spectrometry, non-covalent interactions are preserved in solution and through transfer to the gas phase. This technique can be used to characterize the composition, stoichiometry, and architecture of protein nano-assemblies, such as those observed in vivo or constructed through protein engineering in nanotechnology and synthetic biology. Here we describe an implementation of native mass spectrometry for studying protein-based nanostructures, including membrane proteins. Unambiguous structural details of assemblies can be rapidly determined due to the high resolution and mass accuracy afforded by mass spectrometry measurements including protein nano-assembly stoichiometry, heterogeneity, and ligand binding characteristics.
Key wordsNative mass spectrometry Proteins Membrane proteins Protein nanotechnology
- 22.Kirshenbaum N, Michaelevski I, Sharon M (2010) Analyzing large protein complexes by structural mass spectrometry. J Vis Exp 40:e1954Google Scholar
- 27.Mehmood S, Corradi V, Choudhury HG, Hussain R, Becker P, Axford D, Zirah S, Rebuffat S, Tieleman DP, Robinson CV, Beis K (2016) Structural and functional basis for lipid synergy on the activity of the antibacterial peptide ABC transporter McjD. J Biol Chem 291(41):21656–21668PubMedCrossRefGoogle Scholar