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Cost-Effective Profiling of Mutator Transposon Insertions in Maize by Next-Generation Sequencing

  • Xinyan Zhang
  • Meixia Zhao
  • Damon LischEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2072)

Abstract

Transposable elements can be highly mutagenic because when they transpose they can insert into genes and disrupt their function, a propensity which has been exploited in many organisms to generate tagged mutant alleles. The Mutator (Mu) family transposon is a family of DNA-type transposons in maize with a particularly high duplication frequency, which results in large numbers of new mutations in lineages that carry active Mu elements. Here we describe a rapid and cost-effective Miseq-based Mu transposon profiling pipeline. This method can also be used for identifying flanking sequences of other types of long insertions such as T-DNAs.

Key words

Transposon Mutator Insertion profiling Next-generation sequencing (NGS) Target site duplication Terminal inverted repeats 

Notes

Acknowledgments

This work was supported by the National Science Foundation Grant DBI-1237931 and Purdue Startup Funds to D.L.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Department of Botany and Plant PathologyPurdue UniversityWest LafayetteUSA
  2. 2.Center for Plant BiologyPurdue UniversityWest LafayetteUSA

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