Immobilization of Enzymes and Microorganisms
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Immobilization in polyacrylamide gel is a technique based on the polymerization of acrylamide with N,N′-methylene-bis-acrylamide (Bis) as the cross-linking agent. The degree of cross-linking is controlled by adjusting the ratio of acrylamide to Bis used. Being nonionic in nature, polyacrylamide is the most widely used matrix for entrapping enzymes.