To Perform Biochemical Identification of Microorganism by Nitrogen Source Utilization or Urease Test

  • Aakanchha Jain
  • Richa Jain
  • Sourabh Jain
Part of the Springer Protocols Handbooks book series (SPH)


Urea agar medium contains urea and phenol red. Urease is an enzyme that catalyzes the conversion of urea to CO2 and NH3. Ammonia combines with water to produce ammonium hydroxide, a strong base which increases pH of the medium. Increase in the pH causes phenol red to turn deep pink. This is indicative of a positive reaction for urease.

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© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Aakanchha Jain
    • 1
  • Richa Jain
    • 2
  • Sourabh Jain
    • 3
  1. 1.Bhagyoday Tirth Pharmacy CollegeSagarIndia
  2. 2.Centre for Scientific Research and DevelopmentPeople’s UniversityBhopalIndia
  3. 3.Sagar Institute of Pharmaceutical SciencesSagarIndia

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