To Perform Biochemical Identification of Microorganism by Nitrogen Source Utilization or Urease Test
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Urea agar medium contains urea and phenol red. Urease is an enzyme that catalyzes the conversion of urea to CO2 and NH3. Ammonia combines with water to produce ammonium hydroxide, a strong base which increases pH of the medium. Increase in the pH causes phenol red to turn deep pink. This is indicative of a positive reaction for urease.