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Assessing Rhoptry Secretion in T. gondii

  • Catherine Suarez
  • Melissa B. Lodoen
  • Maryse LebrunEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2071)

Abstract

Rhoptries are key secretory organelles for Toxoplasma gondii invasion. Here, we describe how to assess the ability of T. gondii tachyzoites to secrete their rhoptry contents in vitro.

Key words

Rhoptries Secretion Reporter assay FRET SeCreET 

Notes

Acknowledgments

Our research on rhoptry secretion is supported by the Laboratoire d’Excellence (LabEx) (ParaFrap ANR-11-LABX-0024) and by the Fondation pour la Recherche Médicale (Equipe FRM EQ. 20170336725) to M.L. M.B.L. is supported by NIH R01AI20846. We would like to thank Julien Marcetteau for the graphic design of the figure and Anita Koshy for helpful comments.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Catherine Suarez
    • 1
  • Melissa B. Lodoen
    • 2
    • 3
  • Maryse Lebrun
    • 1
    Email author
  1. 1.UMR 5235 CNRS, Université de MontpellierMontpellierFrance
  2. 2.Department of Molecular Biology and BiochemistryUniversity of California, IrvineIrvineUSA
  3. 3.Institute for ImmunologyUniversity of California, IrvineIrvineUSA

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