Measuring Solute Transport in Toxoplasma gondii Parasites

  • Esther Rajendran
  • Kiaran Kirk
  • Giel G. van DoorenEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2071)


The uptake of host-derived nutrients is key to the growth and survival of Toxoplasma gondii parasites. Nutrients are acquired via solute transporters that localize to the plasma membrane of the parasites. In this chapter, we describe methodology by which the uptake of solutes via plasma membrane transporters may be monitored and characterized. These assays, used here to investigate the uptake of amino acids into parasites, have broad applicability in measuring the uptake of a diverse range of solutes.

Key words

Toxoplasma gondii Transporters Amino acids Plasma membrane 



Work in this area in our laboratories was supported by a grant from the Australian Research Council (DP150102883) to K.K. and G.v.D.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Esther Rajendran
    • 1
  • Kiaran Kirk
    • 1
  • Giel G. van Dooren
    • 1
    Email author
  1. 1.Research School of BiologyAustralian National UniversityActonAustralia

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