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Phenotyping Toxoplasma Invasive Skills by Fast Live Cell Imaging

  • Georgios Pavlou
  • Isabelle TardieuxEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2071)

Abstract

Host cell invasion by Toxoplasma gondii/T. gondii tachyzoites is an obligate but complex multistep process occurring in second-scale. To capture the dynamic nature of the whole entry process requires fast and high-resolution live cell imaging. Recent advances in T. gondii/host cell genome editing and in quantitative live cell imaging—image acquisition and processing included—provide a systematic way to accurately phenotype T. gondii tachyzoite invasive behaviour and to highlight any variation or default from a standard scenario. Therefore, applying these combined strategies allows gaining deeper insights into the complex mechanisms underlying host cell invasion.

Key words

T. gondii tachyzoite Host cell invasion Live cell microscopy Image processing 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  1. 1.Institute for Advanced Biosciences (IAB), Team Membrane Dynamics of Parasite-Host Cell InteractionsCNRS UMR5309, INSERM U1209, Université Grenoble AlpesGrenobleFrance

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