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cDNA Display: A Stable and Simple Genotype–Phenotype Coupling Using a Cell-Free Translation System

  • Hidenao Arai
  • Shigefumi Kumachi
  • Naoto NemotoEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 2070)

Abstract

A cDNA display method was developed based on the mRNA display method to increase its stability and efficiency for the directed evolution of various kinds of peptides and proteins. In this method, the puromycin-linker is a key molecule to realize smart genotype–phenotype coupling. A recently improved puromycin-linker and its use were explained in detail for the in vitro selection of peptides and proteins using the cDNA display method.

Key words

cDNA display mRNA display In vitro selection Directed evolution Puromycin-linker cnvK Photocrosslinking 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Hidenao Arai
    • 1
  • Shigefumi Kumachi
    • 1
  • Naoto Nemoto
    • 1
    • 2
    Email author
  1. 1.Epsilon Molecular Engineering, Inc.SaitamaJapan
  2. 2.Graduate School of Science and EngineeringSaitama UniversitySaitamaJapan

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