Affitins: Ribosome Display for Selection of Aho7c-Based Affinity Proteins

  • Valentina Kalichuk
  • Stanimir Kambarev
  • Ghislaine Béhar
  • Benjamin Chalopin
  • Axelle Renodon-Cornière
  • Barbara Mouratou
  • Frédéric PecorariEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 2070)


Engineered protein scaffolds have made a tremendous contribution to the panel of affinity tools owing to their favorable biophysical properties that make them useful for many applications. In 2007, our group paved the way for using archaeal Sul7d proteins for the design of artificial affinity ligands, so-called Affitins. For many years, Sac7d and Sso7d have been used as molecular basis to obtain binders for various targets. Recently, we characterized their old gifted protein family and identified Aho7c, originating from Acidianus hospitalis, as the shortest member (60 amino-acids) with impressive stability (96.5 °C, pH 0–12). Here, we describe the construction of Aho7c combinatorial libraries and their use for selection of binders by ribosome display.

Key words

Ribosome display In vitro selection Sul7d Aho7c Sac7d Sso7d Affitin 



The authors thank all previous members of the laboratory who helped to develop this protocol. In memoriam of Ghislaine Béhar.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2020

Authors and Affiliations

  • Valentina Kalichuk
    • 1
  • Stanimir Kambarev
    • 1
  • Ghislaine Béhar
    • 1
  • Benjamin Chalopin
    • 1
  • Axelle Renodon-Cornière
    • 1
  • Barbara Mouratou
    • 1
  • Frédéric Pecorari
    • 1
    Email author
  1. 1.CRCINA, INSERM, CNRSUniversité d’Angers, Université de NantesNantesFrance

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