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Snake and Spider Toxins pp 239–268Cite as

Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 2068))

Abstract

Snake and spider envenomation have a considerable impact on public health. Their pathology is induced by a variety of toxins composing the venom which induce cytotoxicity to cells of different organs by several cell death pathways. Described in this chapter are methods in vitro used to assess venoms and toxin-induced cell death using mammalian cell cultures. The chapter is divided into five sections: (1) a brief overview of in vitro cytotoxicity and categories of cell death induced by venoms and toxins; (2) a common method to measure necrotic cell death using lactate dehydrogenase (LDH) release; (3) a flow cytometry method that simultaneously measures necrosis and apoptosis; (4) measurements of nuclear morphology; and (5) measurements of the autophagy following microtubule-associated protein light chain 3 (LC3) expression, by immunoblotting and by fluorescence microscopy of LC3-positive vesicles, to assess the levels of autophagosomes.

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Acknowledgments

Philip Lazarovici holds the Jacob Gitlin Chair in Physiology and is affiliated and supported by the David R Bloom Center for Pharmacy and the Adolph and Klara Brettler Medical Research Center at the Hebrew University of Jerusalem, Israel.

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Authors and Affiliations

  1. Faculty of Medicine, School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel

    Yossi Maatuf, Avi Priel & Philip Lazarovici

Authors
  1. Yossi Maatuf
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  2. Avi Priel
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  3. Philip Lazarovici
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Corresponding author

Correspondence to Philip Lazarovici .

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Editors and Affiliations

  1. Faculty of Medicine, School of Pharmacy, Institute for Drug Research, The Hebrew University of Jerusalem, Jerusalem, Israel

    Avi Priel

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Maatuf, Y., Priel, A., Lazarovici, P. (2020). Measurements of Cell Death Induced by Snake and Spider’s Venoms and Derived Toxins. In: Priel, A. (eds) Snake and Spider Toxins. Methods in Molecular Biology, vol 2068. Humana, New York, NY. https://doi.org/10.1007/978-1-4939-9845-6_13

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  • DOI: https://doi.org/10.1007/978-1-4939-9845-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-4939-9844-9

  • Online ISBN: 978-1-4939-9845-6

  • eBook Packages: Springer Protocols

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